《植物生理学报》 2010, 46(5): 459-465

菊芋组织培养快繁技术的建立

陆杰^1,2, 宋洋^2,3, 王^2,3, 王秀君^2,3, 肖晖^2,3, 王婧¹, 穆艳杰⁴, 李柱刚^2,3,*

1 黑龙江大学生命科学学院生物化学与分子生物学专业, 哈尔滨150080; 2 黑龙江省农业科学院生物技术研究所, 哈尔滨

通信作者：4, 李柱；E-mail: l izhugang@163.com；Tel: 0451-86661 820

摘　要：

以菊芋带芽点的薯盘及带节的幼嫩茎段为外植体, MS为基本培养基, 附加不同种类和浓度的生长调节物质, 研究菊 芋组织培养和快速繁殖的技术环节, 最终筛选出最优技术参数组合, 建立菊芋再生体系。试验结果表明: 茎段外植体是理 想的快速繁殖材料, 正接(形态学下端向下)是最佳的接种方式。芽诱导最佳培养基为 MS+1.0 mg•L-1 6-BA+0.2 mg•L-1 IBA, 诱导率为 95%; 继代增殖最适宜培养基为MS+2.0 mg•L-1 6-BA; 壮苗培养最佳培养基为MS+0.1 mg•L-1 6-BA; 生根适宜培养 基为 MS+0.2 mg•L-1 NAA, 生根率达 100%; 移栽成活率达 95%, 大田种植成活率达 95% 以上。

关键词：菊芋; 外植体; 组织培养; 快速繁殖

收稿：2010-02-08   修定：2010-03-15

资助：黑龙江省科技厅国际合作重点项目(WB07A10)。

Establishment of Tissue Culture and Rapid Propagation Technology of Helianthus tuberosus L.

LU Jie^1,2, SONG Yang^2,3, WANG Xun^2,3, WANG Xiu-Jun^2,3, XIAO Hui^2,3, WANG Jing¹, MU Yan-Jie⁴, LI Zhu-Gang^2,3,*

¹Biochemistry and Molecular Biology of Graduate Academy, College of Life Sciences, Heilongjiang University, Harbin 150080, China; ²Biotechnology Institute, Heilongjiang Academy of Agriculture Sciences, Harbin 150086, China; ³Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Harbin 150086, China; ⁴Microbiology of Graduate Academy, College of Life Sciences, Heilongjiang University, Harbin 150080, China

Corresponding author: LI Zhu-Gang; E-mail: l izhugang@163.com; Tel: 0451-86661 820

Abstract:

Helianthus tuberosus (Jerusalem artichoke) tubers with sprout and shoots with a section of these young stem segments were taken as explants, and were cultured on MS basic medium with several plant growth regulating substances in different concentrations. The technology of tissue culture and rapid propagation on Jerusalem artichoke was researched, and finally filtered out the best combination of technical parameters to establish Jerusalem artichoke regeneration system. The results showed that the optimal explants material for rapid propagation were shoots, positive inoculation (morphology lower down) was the optimal inoculation method. The optimal shoot induction medium was MS+1.0 mg·L^-1 6-BA +0.2 mg·L^-1 IBA with the induction rate of 95%. The optimal subculture medium was MS+2.0 mg·L^-1 6-BA, the optimal strong seedling medium was MS+0.1 mg·L^-1 6-BA. The optimal rooting medium was MS+0.2 mg·L^-1 NAA, the rooting rate reached 100%,transplant survival rate of 95%, field planting survival rate of 95%.

Key words: Helianthus tuberosus; explants; tissue culture; rapid propagation